‪Krister Wiklund‬ - ‪Google Scholar‬

100610 Keira Disp - Swedish Medical Nanoscience Center

Our results not only provide further evidence of the importance of type 1 fimbriae in UTI, but also validate the use … Type 1 fimbriae and adherence to HeLa cells occurred similarly often in all groups of strains. We conclude that P fimbriae, but not type 1 fimbriae or HeLa cell adherence seemed to contribute to the ability of the E. coli strain to colonize the human intestine. Type 1 Fimbriae, a Colonization Factor of Uropathogenic Escherichia coli, Are Controlled by the Metabolic Sensor CRP-cAMP The type 1 fimbriae regulatory protein FimE (RWLH03922), FimF protein precursor (RWLH00227, RWLH01383, RWLH03927), FimG protein precursor (RWLH00226, RWLH03929), FimH protein precursor (RWLH00225, RWLH03930), fimbrin-like protein FimI (RWLH03924), type-1 fimbrial protein A chain precursor (RWLH00230, RWLH03923) and chaperone protein EcpD Isolated type 1 fimbriae inhibited the binding of fimbriated E. coli to purified receptor in a dose- and time-related fashion. The calculated binding affinity was 6 X 10(6) M-1, a value consistent with the low binding affinity expected from previous studies of the agglutination of guinea pig erythrocytes by isolated type 1 fimbriae. 2020-01-13 type 1 fimbriae withthe salivaryglycoprotein wasvisualized as darkly staining bands in the region corresponding to the immobilized salivary glycoprotein on the nitrocellulose strips. Fordirect visualization, the nitrocellulose strip withtrans-ferred salivary glycoprotein was stained with 1% amido Type 1 fimbriae from different genera within Enterobac-teriaceae express MSlectinlike activities that differ in sen-sitivity to saccharides and in sensitivity to hydrophobic moieties(18). Mutant bacteria lacking type 1 fimbriae could not bind to an artificial tooth surface, whereas mutants lacking type 2 fimbriae attach in the same way normal bacteria.

Oral … Very little fimbriae is known of the binding region of type 1 fimbriae. Recently, we observed that dissociated by guanidine hydrochloride retained binding activities suggesting that mannose binding adhesins resided either on the fimbrial subunit or on a minor component of the fimbriae. Type 1 fimbriae mutants of Esoherichia coii 1441 extensive fim homology (Fig. 2, lane 2). Digestion of the DNA with Hpal produces three bands of 7.3, 1.5, and 0.9kbp that co-migrate v^^ith the MG1655 sequences, and a fourth band of approximately 3.3kbp (Fig. 2).

Andrea Puhar - Umeå universitet

2015 – 2016. This project consisted of studying type 1 fimbrie expression in E. coli bacteria when exposed to En beståndsdel av tranbär är fruktos, vilket har varit inblandad i hämningen av E. coli med typ 1 fimbriae (mannos-känslig) (2).

Bakteriologi, Herthelius

2020-01-13 type 1 fimbriae withthe salivaryglycoprotein wasvisualized as darkly staining bands in the region corresponding to the immobilized salivary glycoprotein on the nitrocellulose strips. Fordirect visualization, the nitrocellulose strip withtrans-ferred salivary glycoprotein was stained with 1% amido Type 1 fimbriae from different genera within Enterobac-teriaceae express MSlectinlike activities that differ in sen-sitivity to saccharides and in sensitivity to hydrophobic moieties(18).

2009-02-20 2016-01-05 Type 1 fimbriae are a crucial factor for the virulence of uropathogenic Escherichia coli during the first steps of infection by mediating adhesion to epithelial cells. They are also required for the consequent colonization of the tissues and for invasion of the uroepithelium. 2009-03-13 2014-03-01 2002-08-01 ABSTRACT Type 1 fimbriae are adhesion organelles ex-pressed by many Gram-negative bacteria. They facilitate adherencetomucosalsurfacesandinflammatorycellsinvitro, buttheircontribution tovirulencehasnotbeendefined.This study presents evidence that type 1 fimbriae increase the virulenceofEscherichiacolifortheurinarytractbypromoting Type 1 fimbriae facilitate invasion of uroepithelial cells and the formation of intracellular bacterial communities has been suggested to facilitate bacterial persistence and cystitis in mice (Wright et al., 2007; Anderson et al., 2003) and possibly in humans as well (Rosen et al., 2007). 2019-06-10 2019-05-14 Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
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Previous research has shown that induced, plasmid-based expression of a Pap regulator, papB, and its close homologues can prevent inversion of the fim switch controlling the expression of type 1 fimbriae.

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Influensa och andra luftvägsinfektioner - Region Gävleborg

BMC Microbiol 12:111–111. The occurrence and distribution of fimbrial operons vary significantly among E. coli pathovars and even among the many Salmonella serovars. Therefore, general principles are presented on the basis of detailed discussion of paradigms that have been extensively studied, including Pap, type 1 fimbriae, and curli.

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Hamse Mohamed - Laboratoriespecialist - Cytiva formerly GE

2009-03-13 2014-03-01 2002-08-01 ABSTRACT Type 1 fimbriae are adhesion organelles ex-pressed by many Gram-negative bacteria. They facilitate adherencetomucosalsurfacesandinflammatorycellsinvitro, buttheircontribution tovirulencehasnotbeendefined.This study presents evidence that type 1 fimbriae increase the virulenceofEscherichiacolifortheurinarytractbypromoting Type 1 fimbriae facilitate invasion of uroepithelial cells and the formation of intracellular bacterial communities has been suggested to facilitate bacterial persistence and cystitis in mice (Wright et al., 2007; Anderson et al., 2003) and possibly in humans as well (Rosen et al., 2007). 2019-06-10 2019-05-14 Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs. GO - Molecular function i identical protein binding Source: IntAct Complete GO … 2012-09-01 1999-08-31 Among dozens of different bacterial adhesins, type 1 fimbriae (T1F) are one of the most common adhesive organelles in the members of the Enterobacteriaceae family, including Salmonella spp., and are important virulence factors in pathogenicity. Type 1 fimbriae are composite structures with a relatively rigid 8 nm wide rod tipped by a thin (2.5 nm) more flexible tip fibrillum.